Electrospun Silk Fibroin Nanofibrous Scaffolds with Two-Stage Hydroxyapatite Functionalization for Enhancing the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells
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Title
- Electrospun Silk Fibroin Nanofibrous Scaffolds with Two-Stage Hydroxyapatite Functionalization for Enhancing the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells
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Author(s)
- Eunkyung Ko; Jong Seung Lee; Hyunryung Kim,; Sung Yeun Yang; Dasom Yang; Kisuk Yang; JiYong Lee; Jisoo Shin; Hee Seok Yang; WonHyoung Ryu; Seung-Woo Cho
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Subject
- electrospun silk fibroin scaffolds, hydroxyapatite particles, polydopamine,, ; human adipose-derived mesenchymal stem cells, osteogenesis
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Publication Date
- 2018-03
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Journal
- ACS APPLIED MATERIALS & INTERFACES, v.10, no.9, pp.7614 - 7625
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Publisher
- AMER CHEMICAL SOC
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Abstract
- The development of functional scaffolds with improved osteogenic
potential is important for successful bone formation and mineralization in bone
tissue engineering. In this study, we developed a functional electrospun silk fibroin
(SF) nanofibrous scaffold functionalized with two-stage hydroxyapatite (HAp)
particles, using mussel adhesive-inspired polydopamine (PDA) chemistry. HAp
particles were first incorporated into SF scaffolds during the electrospinning
process, and then immobilized onto the electrospun SF nanofibrous scaffolds
containing HAp via PDA-mediated adhesive chemistry. We obtained two-stage
HAp-functionalized SF nanofibrous scaffolds with improved mechanical properties
and capable of providing a bone-specific physiological microenvironment. The
developed scaffolds were tested for their ability to enhance the osteogenic
differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) in
vitro and repair bone defect in vivo. To boost their ability for bone repair, we
genetically modified hADMSCs with the transcriptional coactivator with PDZbinding
motif (TAZ) via polymer nanoparticle-mediated gene delivery. TAZ is a well-known transcriptional modulator that
activates the osteogenic differentiation of mesenchymal stem cells (MSCs). Two-stage HAp-functionalized SF scaffolds
significantly promoted the osteogenic differentiation of TAZ-transfected hADMSCs in vitro and enhanced mineralized bone
formation in a critical-sized calvarial bone defect model. Our study shows the potential utility of SF scaffolds with nanofibrous
structures and enriched inorganic components in bone tissue engineering.© 2017 American Chemical Society
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URI
- https://pr.ibs.re.kr/handle/8788114/4635
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DOI
- 10.1021/acsami.7b03328
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ISSN
- 1944-8244
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Appears in Collections:
- Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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