Terminal Uridylyltransferases Execute Programmed Clearance of Maternal Transcriptome in Vertebrate Embryos

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Title
Terminal Uridylyltransferases Execute Programmed Clearance of Maternal Transcriptome in Vertebrate Embryos
Author(s)
Hyeshik Chang; Jinah Yeo; Jeong-gyun Kim; Hyunjoon Kim; Jaechul Lim; Mihye Lee; Hyun Ho Kim; Jiyeon Ohk; Hee-Yeon Jeon; Hyunsook Lee; Hosung Jung; Kyu-Won Kim; V. Narry Kim
Publication Date
2018-04
Journal
Molecular Cell, v.70, no.1, pp.72 - 82
Publisher
Elsevier Inc.
Abstract
During the maternal-to-zygotic transition (MZT),maternal RNAs are actively degraded and replaced by newly synthesized zygotic transcripts in a highly coordinated manner. However, it remains largely unknown how maternal mRNA decay is triggered in early vertebrate embryos. Here, through genomewide profiling of RNA abundance and 30 modification, we show that uridylation is induced at the onset of maternal mRNA clearance. The temporal control of uridylation is conserved in vertebrates. When the homologs of terminal uridylyltransferases TUT4 and TUT7 (TUT4/7) are depleted in zebrafish and Xenopus, maternal mRNA clearance is significantly delayed, leading to developmental defects during gastrulation. Short-tailed mRNAs are selectively uridylated by TUT4/7, with the highly uridylated transcripts degraded faster during the MZT than those with unmodified poly(A) tails. Our study demonstrates that uridylation plays a crucial role in timely mRNA degradation, thereby allowing the progression of early development. (c) 2018 Elsevier Inc.
URI
https://pr.ibs.re.kr/handle/8788114/4554
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
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