Correlative three-dimensional fluorescence and refractive index tomography: bridging the gap between molecular specificity and quantitative bioimaging

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Title
Correlative three-dimensional fluorescence and refractive index tomography: bridging the gap between molecular specificity and quantitative bioimaging
Author(s)
Kyoohyun Kim; Wei Sun Park; SangChan Na; Sangbum Kim; Taehong Kim; Won Do HEO; YongKeun Park
Publication Date
2017-12
Journal
Biomedical Optics Express, v.8, no.12, pp.5688 - 5697
Publisher
OPTICAL SOC AMER
Abstract
Optical diffraction tomography (ODT) provides label-free three-dimensional (3D) refractive index (RI) measurement of biological samples. However, due to the nature of the RI values of biological specimens, ODT has limited access to molecular specific information. Here, we present an optical setup combining ODT with three-channel 3D fluorescence microscopy, to enhance the molecular specificity of the 3D RI measurement. The 3D distribution and 3D deconvoluted fluorescence images of HeLa cells and NIH-3T3 cells measured, and the cross-correlative analysis between RI and fluorescence of live cells presented. (C) 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreemen
URI
https://pr.ibs.re.kr/handle/8788114/4458
ISSN
2156-7085
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
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