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식물 노화·수명 연구단
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Prospective Isolation of ISL1 + Cardiac Progenitors from Human ESCs for Myocardial Infarction Therapy

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Title
Prospective Isolation of ISL1 + Cardiac Progenitors from Human ESCs for Myocardial Infarction Therapy
Author(s)
Daehee Hwang; Zaniar Ghazizadeh; Faranak Fattahi; Mehdi Mirzaei; Delger Bayersaikhan; Jaesuk Lee; Sehyun Chae; Hossein Baharvand; Kyunghee Byun; Mehdi Sharifi Tabar; Sara Taleahmad; Shahab Mirshahvaladi; Parisa Shabani; Paul A. Haynes; Nasser Aghdami; Todd Evans; Bonghee Lee; Ghasem Hosseini Salekdeh
Publication Date
2018-03
Journal
Stem Cell Reports, v.10, no.3, pp.848 - 859
Publisher
CELL PRESS
Abstract
The LIM-homeodomain transcription factor ISL1 marks multipotent cardiac progenitors that give rise to cardiac muscle, endothelium, and smooth muscle cells. ISL1+ progenitors can be derived from human pluripotent stem cells, but the inability to efficiently isolate pure populations has limited their characterization. Using a genetic selection strategy, we were able to highly enrich ISL1+ cells derived from human embryonic stem cells. Comparative quantitative proteomic analysis of enriched ISL1+ cells identifiedALCAM(CD166) as a surface marker that enabled the isolation of ISL1+ progenitor cells. ALCAM+/ISL1+ progenitors are multipotent and differentiate into cardiomyocytes,endothelial cells, and smooth muscle cells. Transplantation of ALCAM+ progenitors enhances tissue recovery, restores cardiac function,and improves angiogenesis through activation of AKT-MAPK signaling in a rat model of myocardial infarction, based on cardiac MRI and histology. Our study establishes an efficient method for scalable purification of human ISL1+ cardiac precursor cells for therapeutic applications. (open access)
URI
http://pr.ibs.re.kr/handle/8788114/4441
DOI
10.1016/j.stemcr.2018.01.037
ISSN
2213-6711
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > Journal Papers (저널논문)
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2.Stem Cell Reports_2018_Prospective Isolation of ISL1 + Cardiac Progenitors from Human ESCs for Myocardial Infarction Therapy.pdfDownload

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