Derivation of hypermethylated pluripotent embryonic stem cells with high potency.

Cited 0 time in webofscience Cited 0 time in scopus
217 Viewed 21 Downloaded
Title
Derivation of hypermethylated pluripotent embryonic stem cells with high potency.
Author(s)
Siqin Bao; Walfred WC Tang; Baojiang Wu; Shinseog Kim; Jingyun Li; Lin Li; Toshihiro Kobayashi; Caroline Lee; Yanglin Chen; Mengyi Wei; Shudong Li; Sabine Dietmann; Fuchou Tang; Xihe Li; M Azim Surani
Publication Date
2018-01
Journal
CELL RESEARCH, v.28, no.1, pp.22 - 34
Publisher
INST BIOCHEMISTRY & CELL BIOLOGY
Abstract
Naive hypomethylated embryonic pluripotent stem cells (ESCs) are developmentally closest to the preimplantation epiblast of blastocysts, with the potential to contribute to all embryonic tissues and the germline, excepting the extra-embryonic tissues in chimeric embryos. By contrast, epiblast stem cells (EpiSCs) resembling postimplantation epiblast are relatively more methylated and show a limited potential for chimerism. Here, for the first time, we reveal advanced pluripotent stem cells (ASCs), which are developmentally beyond the pluripotent cells in the inner cell mass but with higher potency than EpiSCs. Accordingly, a single ASC contributes very efficiently to the fetus, germline, yolk sac and the placental labyrinth in chimeras. Since they are developmentally more advanced, ASCs do not contribute to the trophoblast. ASCs were derived from blastocysts in two steps in a chemically defined medium supplemented with Activin A and basic fibroblast growth factor, followed by culturing in ABCL medium containing ActA, BMP4, CHIR99021 and leukemia inhibitory factor. Notably, ASCs exhibit a distinct transcriptome with the expression of both naive pluripotency genes, as well as mesodermal somatic genes; Eomes, Eras, Tdgf1, Evx1, hand1, Wnt5a and distinct repetitive elements. Conversion of established ESCs to ASCs is also achievable. Importantly, ASCs exhibit a stable hypermethylated epigenome and mostly intact imprints as compared to the hypomethylated inner cell mass of blastocysts and naive ESCs. Properties of ASCs suggest that they represent cells at an intermediate cellular state between the naive and primed states of pluripotency. © The Author(s) 2017
URI
https://pr.ibs.re.kr/handle/8788114/4253
ISSN
1001-0602
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > Journal Papers (저널논문)
Files in This Item:
cr2017134.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse