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Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets

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Title
Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets
Author(s)
Inho Nam; Hong Gil Nam; Richard N. Zare
Publication Date
2018-01
Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-PHYSICAL SCIENCES, v.115, no.1, pp.36 - 40
Publisher
NATL ACAD SCIENCES
Abstract
Aqueous microdroplets (<1.3 µm in diameter on average) containing 15 mM D-ribose, 15 mM phosphoric acid, and 5 mM of a nucleobase (uracil, adenine, cytosine, or hypoxanthine) are electrosprayed from a capillary at +5 kV into a mass spectrometer at room temperature and 1 atm pressure with 3 mM divalent magnesium ion (Mg2+) as a catalyst. Mass spectra show the formation of ribonucleosides that comprise a four-letter alphabet of RNA with a yield of 2.5% of uridine (U), 2.5% of adenosine (A), 0.7% of cytidine (C), and 1.7% of inosine (I) during the flight time of ∼50 µs. In the case of uridine, no catalyst is required. An aqueous solution containing guanine cannot be generated under the same conditions given the extreme insolubility of guanine in water. However, inosine can base pair with cytidine and thus substitute for guanosine. Thus, a full set of ribonucleosides to generate the purine–pyrimidine base pairs A-U and I-C are spontaneously generated in aqueous microdroplets under similar mild conditions. © Copyright 2017 Elsevier B.V., All rights reserved.
URI
http://pr.ibs.re.kr/handle/8788114/4125
DOI
10.1073/pnas.1718559115
ISSN
0027-8424
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > Journal Papers (저널논문)
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