Highly Efficient and Rapid Neural Differentiation of Mouse Embryonic Stem Cells Based on Retinoic Acid Encapsulated Porous Nanoparticle

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Title
Highly Efficient and Rapid Neural Differentiation of Mouse Embryonic Stem Cells Based on Retinoic Acid Encapsulated Porous Nanoparticle
Author(s)
Se-Jin Park; Seongchan Kim; Sung-Yon Kim; Noo Li Jeon; Joon Myong Song; Cheolhee Won; Dal-Hee Min
Publication Date
2017-10
Journal
ACS APPLIED MATERIALS & INTERFACES, v.9, no.40, pp.34634 - 34640
Publisher
AMER CHEMICAL SOC
Abstract
An improved cell conversion strategy for neural differentiation of mouse embryonic stem (mES) cells is developed by incorporating functionalized mesoporous silica nanoparticle (MSN) as an efficient delivery carrier of retinoic acid (RA), which is a pleiotropic factor required for initiation of neural differentiation. Traditional RA-mediated neural differentiation methods required either preactivation of the cells to the differentiating state by embryoid body (EB) formation or repetitive treatment of the differentiation factor. Our modified cell conversion system involves only singular treatment of the RA/MSN complex, which simplified the whole process and accelerated neural induction to be finished within 6 days with high quality. With our new method, neural cells were successfully derived from mES cells with stable expression of neurite marker gene. © 2017 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/4005
ISSN
1944-8244
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
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