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식물 노화·수명 연구단
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Transcriptional regulatory networks underlying the reprogramming of spermatogonial stem cells to multipotent stem cells

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Title
Transcriptional regulatory networks underlying the reprogramming of spermatogonial stem cells to multipotent stem cells
Author(s)
Hoe-Su Jeong; Jinhyuk Bhin; Hyung Joon Kim; Daehee Hwang; Dong Ryul Lee; Kye-Seong Kim
Publication Date
2017-04
Journal
EXPERIMENTAL AND MOLECULAR MEDICINE, v.49, no.4, pp.e315 -
Publisher
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Abstract
Spermatogonial stem cells (SSCs) are germline stem cells located along the basement membrane of seminiferous tubules in testes. Recently, SSCs were shown to be reprogrammed into multipotent SSCs (mSSCs). However, both the key factors and biological networks underlying this reprogramming remain elusive. Here, we present transcriptional regulatory networks (TRNs) that control cellular processes related to the SSC-to-mSSC reprogramming. Previously, we established intermediate SSCs (iSSCs) undergoing the transition to mSSCs and generated gene expression profiles of SSCs, iSSCs and mSSCs. By comparing these profiles, we identified 2643 genes that were up-regulated during the reprogramming process and 15 key transcription factors (TFs) that regulate these genes. Using the TF-target relationships, we developed TRNs describing how these TFs regulate three pluripotency-related processes (cell proliferation, stem cell maintenance and epigenetic regulation) during the reprogramming. The TRNs showed that 4 of the 15 TFs (Oct4/Pou5f1, Cux1, Zfp143 and E2f4) regulated cell proliferation during the early stages of reprogramming, whereas 11 TFs (Oct4/Pou5f1, Foxm1, Cux1, Zfp143, Trp53, E2f4, Esrrb, Nfyb, Nanog, Sox2 and Klf4) regulated the three pluripotency-related processes during the late stages of reprogramming. Our TRNs provide a model for the temporally coordinated transcriptional regulation of pluripotency-related processes during the SSC-to-mSSC reprogramming, which can be further tested in detailed functional studies. (c) 2017 KSBMB.
URI
http://pr.ibs.re.kr/handle/8788114/3720
DOI
10.1038/emm.2017.2
ISSN
1226-3613
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > Journal Papers (저널논문)
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