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분자분광학및동력학연구단
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Structure determination of the C-terminal fragment of yeast Ski7 using twinned crystal data

DC Field Value Language
dc.contributor.authorSi Hoon Park-
dc.contributor.authorYong-Boo Kuk-
dc.contributor.authorJi-Young Lee-
dc.contributor.authorByeong-Cheon Jeong-
dc.contributor.authorHyun Kyu Song-
dc.date.available2017-06-05T04:50:55Z-
dc.date.created2017-06-01-
dc.date.issued2017-03-
dc.identifier.issn2288-6982-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/3597-
dc.description.abstractThe structure determination using twinned crystals is challenging although several algorithms have been developed for detwinning the X-ray data. Our crystal of the C-terminal domain 2 and 3 of Ski7 (Ski7-D2/3), a key part of non-stop mRNA decay has a perfect twin with the twin operator [h, -h-k, -l]. Many different efforts for phasing with multiple anomalous dispersion techniques using selenomethionine substituted wild-type and mutant proteins were not successful and the phases were obtained through the molecular replacement method using recently reported structure of C-terminal GTPase domain of Ski7 from Saccharomyces cerevisiae. The overall structure of Ski7-D2/3 is very similar to that of the corresponding domain of ribosome-associated GTPases including eIF5B, eEF1α, and eRF3. Domains 2 and 3 form a β-barrel structure containing several structurally deviated long connecting loops. Although the linker between domain 2 and 3 is very flexible, the relative orientation between them is virtually the same among all structures, showing that the Ski7-D2/3 does not show major conformational movement upon contacting with G domain. © 2017 Biodesign-
dc.language영어-
dc.publisherBiodesign-
dc.titleStructure determination of the C-terminal fragment of yeast Ski7 using twinned crystal data-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.rimsid59558ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorHyun Kyu Song-
dc.identifier.bibliographicCitationBiodesign, v.5, no.1, pp.12 - 23-
dc.relation.isPartOfBiodesign-
dc.citation.titleBiodesign-
dc.citation.volume5-
dc.citation.number1-
dc.citation.startPage12-
dc.citation.endPage23-
dc.description.journalClass2-
dc.description.journalClass2-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClasskci-
dc.description.journalRegisteredClassother-
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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