Efficient method for whole-cell recording in freely moving rodents using ultraviolet-cured collar-based pipette stabilization

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Title
Efficient method for whole-cell recording in freely moving rodents using ultraviolet-cured collar-based pipette stabilization
Author(s)
Doyun Lee; Albert K. Lee
Publication Date
2017-04
Journal
Cold Spring Harbor Protocols, v.2017, no.4, pp.281 - 288
Publisher
Cold Spring Harbor Laboratory Press
Abstract
Whole-cell recording is a key technique for investigating synaptic and cellular mechanisms underlying various brain functions. However, because of its high sensitivity to mechanical disturbances, applying the whole-cell recording method to freely moving animals has been challenging. Here, we describe a technique for obtaining such recordings in freely moving, drug-free animals with a high success rate. This technique involves three major steps: obtaining a whole-cell recording from awake head-fixed animals, reliable and efficient stabilization of the pipette with respect to the animal’s head using an ultraviolet (UV)-transparent collar and UV-cured adhesive, and rapid release of the animal from head fixation without loss of the recording. This technique has been successfully applied to obtain intracellular recordings from the hippocampus of freely moving rats and mice exploring a spatial environment, and should be generally applicable to other brain areas in animals engaged in a variety of natural behaviors. © 2017 Cold Spring Harbor Laboratory Press
URI
https://pr.ibs.re.kr/handle/8788114/3582
ISSN
1940-3402
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
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