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Adenylation of Maternally Inherited MicroRNAs by Wispy

Cited 52 time in webofscience Cited 53 time in scopus
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Title
Adenylation of Maternally Inherited MicroRNAs by Wispy
Author(s)
Mihye Lee; Yeon Choi; Kijun Kim; Hua Jin; Jaechul Lim; Tuan Anh Nguyen; Jihye Yang; Minsun Jeong; Antonio J. Giraldez; Hui Yang; Dinshaw J. Patel; V. Narry Kim
Subject
MESSENGER-RNA TRANSLATION, ; TO-ZYGOTIC TRANSITION, ; POLY(A) POLYMERASE, ; CYTOPLASMIC POLYADENYLATION, ; EGG ACTIVATION, ; OOCYTE MATURATION, ; EMERGING ROLES, ; EARLY EMBRYOS, ; PRE-MICRORNA, ; DROSOPHILA
Publication Date
2014-12
Journal
MOLECULAR CELL, v.56, no.5, pp.696 - 707
Publisher
CELL PRESS
Abstract
Early development depends heavily on accurate control of maternally inherited mRNAs, and yet it remains unknown how maternal microRNAs are regulated during maternal-to-zygotic transition (MZT). We here find that maternal microRNAs are highly adenylated at their 3′ ends in mature oocytes and early embryos. Maternal microRNA adenylation is widely conserved in fly, sea urchin, and mouse. We identify Wispy, a noncanonical poly(A) polymerase, as the enzyme responsible for microRNA adenylation in flies. Knockout of wispy abrogates adenylation and results in microRNA accumulation in eggs, whereas overexpression of Wispy increases adenylation and reduces microRNA levels in S2 cells. Wispy interacts with Ago1 through protein-protein interaction, which may allow the effective and selective adenylation of microRNAs. Thus, adenylation may contribute to the clearance of maternally deposited microRNAs during MZT. Our work provides mechanistic insights into the regulation of maternal microRNAs and illustrates the importance of RNA tailing in development. ⓒ2014 Elsevier Inc.
URI
https://pr.ibs.re.kr/handle/8788114/3577
DOI
10.1016/j.molcel.2014.10.011
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
PIIS1097276514007953.pdfDownload

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