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Substituent Effects in BODIPY in Live Cell Imaging

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Title
Substituent Effects in BODIPY in Live Cell Imaging
Author(s)
Sandip V. Mulay; Yudhistira T.; Choi M.; Youngsam Kim; Kim J.; Jang Y.J.; Jon S.; David G. Churchill
Subject
aggregation induced emission, ; bodipy, ; fluorescent probes, ; hypochlorous acid, ; lipids
Publication Date
2016-12
Journal
CHEMISTRY-AN ASIAN JOURNAL, v.11, no.24, pp.3598 - 3605
Publisher
WILEY-V C H VERLAG GMBH
Abstract
Small-molecule organoselenium-based fluorescent probes possess great capacity in understanding biological processes through the detection of various analytes such as reactive oxygen/nitrogen species (ROS/RNS), biothiols (cysteine, homocysteine and glutathione), lipid droplets, etc. Herein, we present how substituents on the BODIPY system play a significant part in the detection of biologically important analytes for in vitro conditions and live cell imaging studies. The fluorescence of the probe was quenched by 2-chloro and 6-phenyl selenium groups; the probe shows high selectivity with NaOCl among other ROS/RNS, and gives a turn-on response. The maximum fluorescence intensity is attained within ≈1–2 min with a low detection limit (19.6 nm), and shows a ≈110-fold fluorescence enhancement compared to signals generated for other ROS/RNS. Surprisingly, in live cell experiments, the probe specifically located and accumulated in lipid droplets, and showed a fluorescence turn-on response. We believe this turn-on response occurred because of aggregation-induced emission (AIE), which surprisingly occurred only by introducing one lipophilic mesityl group at the meso position of the BODIPY. © 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinhei
URI
https://pr.ibs.re.kr/handle/8788114/3329
DOI
10.1002/asia.201601400
ISSN
1861-4728
Appears in Collections:
Center for Catalytic Hydrocarbon Functionalizations(분자활성 촉매반응 연구단) > 1. Journal Papers (저널논문)
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