BROWSE

Related Scientist

Researcher

Sandip Mulay
분자활성 촉매반응 연구단
more info

Substituent Effects in BODIPY in Live Cell Imaging

Cited 8 time in webofscience Cited 0 time in scopus
352 Viewed 46 Downloaded
Title
Substituent Effects in BODIPY in Live Cell Imaging
Author(s)
Sandip V. Mulay; Yudhistira T.; Choi M.; Youngsam Kim; Kim J.; Jang Y.J.; Jon S.; David G. Churchill
Publication Date
2016-12
Journal
CHEMISTRY-AN ASIAN JOURNAL, v.11, no.24, pp.3598 - 3605
Publisher
WILEY-V C H VERLAG GMBH
Abstract
Small-molecule organoselenium-based fluorescent probes possess great capacity in understanding biological processes through the detection of various analytes such as reactive oxygen/nitrogen species (ROS/RNS), biothiols (cysteine, homocysteine and glutathione), lipid droplets, etc. Herein, we present how substituents on the BODIPY system play a significant part in the detection of biologically important analytes for in vitro conditions and live cell imaging studies. The fluorescence of the probe was quenched by 2-chloro and 6-phenyl selenium groups; the probe shows high selectivity with NaOCl among other ROS/RNS, and gives a turn-on response. The maximum fluorescence intensity is attained within ≈1–2 min with a low detection limit (19.6 nm), and shows a ≈110-fold fluorescence enhancement compared to signals generated for other ROS/RNS. Surprisingly, in live cell experiments, the probe specifically located and accumulated in lipid droplets, and showed a fluorescence turn-on response. We believe this turn-on response occurred because of aggregation-induced emission (AIE), which surprisingly occurred only by introducing one lipophilic mesityl group at the meso position of the BODIPY. © 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinhei
URI
https://pr.ibs.re.kr/handle/8788114/3329
ISSN
1861-4728
Appears in Collections:
Center for Catalytic Hydrocarbon Functionalizations(분자활성 촉매반응 연구단) > Journal Papers (저널논문)
Files in This Item:
Substituent Effects in BODIPY in Live Cell Imaging.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse