Substituent Effects in BODIPY in Live Cell Imaging
DC Field | Value | Language |
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dc.contributor.author | Sandip V. Mulay | - |
dc.contributor.author | Yudhistira T. | - |
dc.contributor.author | Choi M. | - |
dc.contributor.author | Youngsam Kim | - |
dc.contributor.author | Kim J. | - |
dc.contributor.author | Jang Y.J. | - |
dc.contributor.author | Jon S. | - |
dc.contributor.author | David G. Churchill | - |
dc.date.available | 2017-01-26T05:17:02Z | - |
dc.date.created | 2017-01-19 | - |
dc.date.issued | 2016-12 | - |
dc.identifier.issn | 1861-4728 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/3329 | - |
dc.description.abstract | Small-molecule organoselenium-based fluorescent probes possess great capacity in understanding biological processes through the detection of various analytes such as reactive oxygen/nitrogen species (ROS/RNS), biothiols (cysteine, homocysteine and glutathione), lipid droplets, etc. Herein, we present how substituents on the BODIPY system play a significant part in the detection of biologically important analytes for in vitro conditions and live cell imaging studies. The fluorescence of the probe was quenched by 2-chloro and 6-phenyl selenium groups; the probe shows high selectivity with NaOCl among other ROS/RNS, and gives a turn-on response. The maximum fluorescence intensity is attained within ≈1–2 min with a low detection limit (19.6 nm), and shows a ≈110-fold fluorescence enhancement compared to signals generated for other ROS/RNS. Surprisingly, in live cell experiments, the probe specifically located and accumulated in lipid droplets, and showed a fluorescence turn-on response. We believe this turn-on response occurred because of aggregation-induced emission (AIE), which surprisingly occurred only by introducing one lipophilic mesityl group at the meso position of the BODIPY. © 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinhei | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | aggregation induced emission | - |
dc.subject | bodipy | - |
dc.subject | fluorescent probes | - |
dc.subject | hypochlorous acid | - |
dc.subject | lipids | - |
dc.title | Substituent Effects in BODIPY in Live Cell Imaging | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000392511500019 | - |
dc.identifier.scopusid | 2-s2.0-85006024515 | - |
dc.identifier.rimsid | 58402 | ko |
dc.date.tcdate | 2018-10-01 | - |
dc.contributor.affiliatedAuthor | Sandip V. Mulay | - |
dc.contributor.affiliatedAuthor | Youngsam Kim | - |
dc.contributor.affiliatedAuthor | David G. Churchill | - |
dc.identifier.doi | 10.1002/asia.201601400 | - |
dc.identifier.bibliographicCitation | CHEMISTRY-AN ASIAN JOURNAL, v.11, no.24, pp.3598 - 3605 | - |
dc.citation.title | CHEMISTRY-AN ASIAN JOURNAL | - |
dc.citation.volume | 11 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 3598 | - |
dc.citation.endPage | 3605 | - |
dc.date.scptcdate | 2018-10-01 | - |
dc.description.wostc | 8 | - |
dc.description.scptc | 8 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | aggregation induced emission | - |
dc.subject.keywordAuthor | bodipy | - |
dc.subject.keywordAuthor | fluorescent probes | - |
dc.subject.keywordAuthor | hypochlorous acid | - |
dc.subject.keywordAuthor | lipids | - |