Cas-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9

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Title
Cas-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9
Author(s)
Jeongbin Park; Jin-Soo Kim; Sangsu Bae
Publication Date
2016-07
Journal
BIOINFORMATICS, v.32, no.13, pp.2017 - 2023
Publisher
OXFORD UNIV PRESS
Abstract
Motivation: CRISPR-derived RNA guided endonucleases (RGENs) have been widely used for both gene knockout and knock-in at the level of single or multiple genes. RGENs are now available for forward genetic screens at genome scale, but single guide RNA (sgRNA) selection at this scale is difficult. (C) The Author 2016. Published by Oxford University Press. Results: We develop an online tool, Cas-Database, a genome-wide gRNA library design tool for Cas9 nucleases from Streptococcus pyogenes (SpCas9). With an easy-to-use web interface, Cas-Database allows users to select optimal target sequences simply by changing the filtering conditions. Furthermore, it provides a powerful way to select multiple optimal target sequences from thousands of genes at once for the creation of a genome-wide library. Cas-Database also provides a web application programming interface (web API) for advanced bioinformatics users.
URI
https://pr.ibs.re.kr/handle/8788114/3142
ISSN
1367-4803
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
1602_배상수_김진수_Bioinformatics.pdfDownload

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