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Real-Time Imaging Reveals Properties of Glutamate-Induced Arc/Arg 3.1 Translation in Neuronal Dendrites

Cited 33 time in webofscience Cited 36 time in scopus
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Title
Real-Time Imaging Reveals Properties of Glutamate-Induced Arc/Arg 3.1 Translation in Neuronal Dendrites
Author(s)
Youn Na; Sungjin Park; Changhee Lee; Dong-Kyu Kim; Joo Min Park; Shanthini Sockanathan; Richard L. Huganir; Paul F. Worley
Publication Date
2016-08
Journal
NEURON, v.91, no.3, pp.561 - 573
Publisher
CELL PRESS
Abstract
The immediate early gene Arc (also Arg3.1) produces rapid changes in synaptic properties that are linked to de novo translation. Here we develop a novel translation reporter that exploits the rapid maturation and “flash” kinetics of Gaussia luciferase (Gluc) to visualize Arc translation. Following glutamate stimulation, discrete Arc-Gluc bioluminescent flashes representing sites of de novo translation are detected within 15 s at distributed sites in dendrites, but not spines. Flashes are episodic, lasting ∼20 s, and may be unitary or repeated at ∼minute intervals at the same sites. Analysis of flash amplitudes suggests they represent the quantal product of one or more polyribosomes, while inter-flash intervals appear random, suggesting they arise from a stochastic process. Surprisingly, glutamate-induced translation is dependent on Arc open reading frame. Combined observations support a model in which stalled ribosomes are reactivated to rapidly generate Arc protein. © 2016 Elsevier Inc.
URI
https://pr.ibs.re.kr/handle/8788114/3061
DOI
10.1016/j.neuron.2016.06.017
ISSN
0896-6273
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > 1. Journal Papers (저널논문)
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