Knockout of the ribonuclease inhibitor gene leaves human cells vulnerable to secretory ribonucleases

Cited 4 time in webofscience Cited 0 time in scopus
434 Viewed 24 Downloaded
Title
Knockout of the ribonuclease inhibitor gene leaves human cells vulnerable to secretory ribonucleases
Author(s)
Sydney P. Thomas; Eunji Kim; Jin-Soo Kim; Ronald T. Raines
Publication Date
2016-11
Journal
BIOCHEMISTRY, v.55, no.46, pp.6359 - 6362
Publisher
AMER CHEMICAL SOC
Abstract
Ribonuclease inhibitor (RNH1) is a cytosolic protein that binds with femtomolar affinity to human ribonuclease 1 (RNase 1) and homologous secretory ribonucleases. RNH1 contains 32 cysteine residues and has been implicated as an antioxidant. Here, we use CRISPR-Cas9 to knock out RNH1 in HeLa cells. We find that cellular RNH1 affords marked protection from the lethal ribonucleolytic activity of RNase 1 but not from oxidants. We conclude that RNH1 protects cytosolic RNA from invading ribonucleases. © 2016 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/3037
ISSN
0006-2960
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
김은지_김진수_Ebiochem.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse