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복잡계자기조립연구단
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Coordinative Amphiphiles as Tunable siRNA Transporters

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dc.contributor.authorJin Bum Kim-
dc.contributor.authorYeong Mi Lee-
dc.contributor.authorJooyeon Ryu-
dc.contributor.authorEunji Lee-
dc.contributor.authorWon Jong Kim-
dc.contributor.authorGyochang Keum-
dc.contributor.authorEun Kyoung Bang-
dc.date.available2016-11-29T08:18:51Z-
dc.date.created2016-09-20-
dc.date.issued2016-08-
dc.identifier.issn1043-1802-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/2991-
dc.description.abstractIn this study, we developed coordinative amphiphiles for use as novel siRNA transporters. As a modification of a conventional cationic lipid structure, we replaced the cationic head with zinc(II)-dipicolylamine complex (Zn/DPA) as a phosphate directing group, and used various membrane-directing groups in the place of the hydrophobic tails. These simple amphiphiles are readily synthesized and easy to modify. The Zn/DPA head groups bind to the phosphate backbones of siRNAs, and to our surprise, they prevented the enzymatic degradation of siRNAs by RNase A. Interestingly, the Zn/DPA head itself exhibited moderate transfection efficiency, and its combination with a membrane-directing group-oleoyl (CA1), pyrenebutyryl (CA2), or biotin (CA3)-enhanced the delivery efficiency without imparting significant cytotoxicity. Notably, the uptake pathway was tunable depending on the nature of the membrane-directing group. CA1 delivered siRNAs mainly through caveolae-mediated endocytosis, and CA2 through clathrin- and caveolin-independent endocytosis; CA3 recruited siRNAs specifically into biotin receptor-positive HepG2 cells through receptor-mediated endocytosis. Thus, it appears possible to develop tunable siRNA transporters simply by changing the membrane-directing parts. These are the first examples of amphiphilic siRNA transporters accompanying coordinative interactions between the amphiphiles and siRNAs. © 2016 American Chemical Society-
dc.description.uri1-
dc.language영어-
dc.publisherAMER CHEMICAL SOC-
dc.titleCoordinative Amphiphiles as Tunable siRNA Transporters-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000381716200011-
dc.identifier.scopusid2-s2.0-84983450676-
dc.identifier.rimsid56403ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorYeong Mi Lee-
dc.contributor.affiliatedAuthorWon Jong Kim-
dc.identifier.doi10.1021/acs.bioconjchem.6b00260-
dc.identifier.bibliographicCitationBIOCONJUGATE CHEMISTRY, v.27, no.8, pp.1850 - 1856-
dc.citation.titleBIOCONJUGATE CHEMISTRY-
dc.citation.volume27-
dc.citation.number8-
dc.citation.startPage1850-
dc.citation.endPage1856-
dc.date.scptcdate2018-10-01-
dc.description.scptc0-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusCELL-PENETRATING PEPTIDES-
dc.subject.keywordPlusDELIVERY-SYSTEM-
dc.subject.keywordPlusGENE DELIVERY-
dc.subject.keywordPlusPLASMID DNA-
dc.subject.keywordPlusBILAYER-MEMBRANES-
dc.subject.keywordPlusIN-VIVO-
dc.subject.keywordPlusINTRACELLULAR DELIVERY-
dc.subject.keywordPlusMOLECULAR RECOGNITION-
dc.subject.keywordPlusMEDIATED ENDOCYTOSIS-
dc.subject.keywordPlusPROTEIN TRANSDUCTION-
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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