DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

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Title
DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins
Author(s)
Kwangryul Baek; Duk Hyoung Kim; Jooyeon Jeong; Sang Jun Sim; Anastasios Melis; Jin-Soo Kim; EonSeon Jin; Sangsu Bae
Publication Date
2016-07
Journal
SCIENTIFIC REPORTS, v.6, no., pp.30620 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Microalgae are versatile organisms capable of converting CO 2, H 2 O, and sunlight into fuel and chemicals for domestic and industrial consumption. Thus, genetic modifications of microalgae for enhancing photosynthetic productivity, and biomass and bio-products generation are crucial for both academic and industrial applications. However, targeted mutagenesis in microalgae with CRISPR-Cas9 is limited. Here we report, a one-step transformation of Chlamydomonas reinhardtii by the DNA-free CRISPR-Cas9 method rather than plasmids that encode Cas9 and guide RNAs. Outcome was the sequential CpFTSY and ZEP two-gene knockout and the generation of a strain constitutively producing zeaxanthin and showing improved photosynthetic productivity © The Author(s) 2016
URI
https://pr.ibs.re.kr/handle/8788114/2810
ISSN
2045-2322
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
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