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Optogenetic toolkit reveals the role of Ca2+ sparklets in coordinated cell migration

Cited 22 time in webofscience Cited 26 time in scopus
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Title
Optogenetic toolkit reveals the role of Ca2+ sparklets in coordinated cell migration
Author(s)
Jin Man Kim; Minji Lee; Nury, Kim; Won Do Heo
Publication Date
2016-05
Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.113, no.21, pp.5952 - 5957
Publisher
NATL ACAD SCIENCES
Abstract
Cell migration is controlled by various Ca2+ signals. Local Ca2+ signals, in particular, have been identified as versatile modulators of cell migration because of their spatiotemporal diversity. However, little is known about how local Ca2+ signals coordinate between the front and rear regions in directionally migrating cells. Here, we elucidate the spatial role of local Ca2+ signals in directed cell migration through combinatorial application of an optogenetic toolkit. An optically guided cell migration approach revealed the existence of Ca2+ sparklets mediated by L-type voltage-dependent Ca2+ channels in the rear part of migrating cells. Notably, we found that this locally concentrated Ca2+ influx acts as an essential transducer in establishing a global front-to-rear increasing Ca2+ gradient. This asymmetrical Ca2+ gradient is crucial for maintaining front-rear morphological polarity by restricting spontaneous lamellipodia formation in the rear part of migrating cells. Collectively, our findings demonstrate a clear link between local Ca2+ sparklets and front-rear coordination during directed cell migration.
URI
https://pr.ibs.re.kr/handle/8788114/2605
DOI
10.1073/pnas.1518412113
ISSN
0027-8424
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
201605 PNAS.pdfDownload

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