Optogenetic toolkit reveals the role of Ca2+ sparklets in coordinated cell migration

DC Field Value Language
dc.contributor.authorJin Man Kim-
dc.contributor.authorMinji Lee-
dc.contributor.authorNury Kim-
dc.contributor.authorWon Do Heo-
dc.date.available2016-07-05T06:36:16Z-
dc.date.created2016-06-20-
dc.date.issued2016-05-
dc.identifier.issn0027-8424-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/2605-
dc.description.abstractCell migration is controlled by various Ca2+ signals. Local Ca2+ signals, in particular, have been identified as versatile modulators of cell migration because of their spatiotemporal diversity. However, little is known about how local Ca2+ signals coordinate between the front and rear regions in directionally migrating cells. Here, we elucidate the spatial role of local Ca2+ signals in directed cell migration through combinatorial application of an optogenetic toolkit. An optically guided cell migration approach revealed the existence of Ca2+ sparklets mediated by L-type voltage-dependent Ca2+ channels in the rear part of migrating cells. Notably, we found that this locally concentrated Ca2+ influx acts as an essential transducer in establishing a global front-to-rear increasing Ca2+ gradient. This asymmetrical Ca2+ gradient is crucial for maintaining front-rear morphological polarity by restricting spontaneous lamellipodia formation in the rear part of migrating cells. Collectively, our findings demonstrate a clear link between local Ca2+ sparklets and front-rear coordination during directed cell migration.-
dc.languageENG-
dc.publisherNATL ACAD SCIENCES-
dc.titleOptogenetic toolkit reveals the role of Ca2+ sparklets in coordinated cell migration-
dc.typeArticle-
dc.type.rimsA-
dc.identifier.wosid000376779900060-
dc.identifier.scopusid2-s2.0-84969858875-
dc.description.wostc9-
dc.date.tcdate2018-10-01-
dc.date.scptcdate2018-10-01-
dc.subject.keywordActin cytoskeleton-
dc.subject.keywordCalcium signaling-
dc.subject.keywordCell migration-
dc.subject.keywordCell polarity-
dc.subject.keywordOptogenetics-
dc.contributor.affiliatedAuthorNury Kim-
dc.contributor.affiliatedAuthorWon Do Heo-
dc.identifier.bibliographicCitationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.113, no.21, pp.5952 - 5957-
dc.description.scptc9-
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
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