Selective two-photon collagen crosslinking in situ measured by Brillouin microscopy

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Title
Selective two-photon collagen crosslinking in situ measured by Brillouin microscopy
Author(s)
Sheldon J. J. Kwok; Ivan A. Kuznetsov; Moonseok Kim; Myunghwan Choi; Giuliano Scarcelli; Seok Hyun Yun
Publication Date
2016-05
Journal
OPTICA, v.3, no.5, pp.469 - 472
Publisher
OPTICAL SOC AMER
Abstract
Two-photon polymerization has enabled the precise microfabrication of three-dimensional structures with applications spanning from photonic microdevices, drug delivery systems, and cellular scaffolds. We present two-photon collagen crosslinking (2P-CXL) of intact corneal tissue using riboflavin and femtosecond laser irradiation. The collagen fiber orientations and photobleaching were characterized by second harmonic generation and two-photon fluorescence imaging, respectively. The measurement of local changes in longitudinal mechanical moduli with confocal Brillouin microscopy enabled the visualization of the cross-linked pattern without the perturbation of the surrounding non-irradiated regions. 2P-CXL-induced stiffening was comparable to that achieved with conventional one-photon CXL. Our results demonstrate the ability to selectively stiffen biological tissue in situ at high spatial resolution, with broad implications in ophthalmology, laser surgery, and tissue engineering. (C) 2016 Optical Society of America
URI
https://pr.ibs.re.kr/handle/8788114/2573
ISSN
2334-2536
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > Journal Papers (저널논문)
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