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A soft, transparent, freely accessible cranial window for chronic imaging and electrophysiology

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Title
A soft, transparent, freely accessible cranial window for chronic imaging and electrophysiology
Author(s)
Chaejeong Heo; Hyejin Park; Yong-Tae Kim; Eunha Baeg; Yong Ho Kim; Seong-Gi Kim; Minah Suh
Publication Date
2016-06
Journal
SCIENTIFIC REPORTS, v.6, no., pp.27818 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Chronic in vivo imaging and electrophysiology are important for better understanding of neural functions and circuits. We introduce the new cranial window using soft, penetrable, elastic, and transparent, silicone-based polydimethylsiloxane (PDMS) as a substitute for the skull and dura in both rats and mice. The PDMS can be readily tailored to any size and shape to cover large brain area. Clear and healthy cortical vasculatures were observed up to 15 weeks post-implantation. Real-time hemodynamic responses were successfully monitored during sensory stimulation. Furthermore, the PDMS window allowed for easy insertion of microelectrodes and micropipettes into the cortical tissue for electrophysiological recording and chemical injection at any location without causing any fluid leakage. Longitudinal two-photon microscopic imaging of Cx3Cr1+/− GFP transgenic mice was comparable with imaging via a conventional glass-type cranial window, even immediately following direct intracortical injection. This cranial window will facilitate direct probing and mapping for long-term brain studies.
URI
http://pr.ibs.re.kr/handle/8788114/2566
DOI
10.1038/srep27818
ISSN
2045-2322
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > Journal Papers (저널논문)
Files in This Item:
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