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Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9 Highly Cited Paper

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Title
Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9
Author(s)
Taeyoung Koo; Jungjoon Lee; Jin Soo Kim
Publication Date
2015-06
Journal
MOLECULES AND CELLS, v.38, no.6, pp.475 - 481
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Abstract
Programmable nucleases, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) repurposed from the type II clustered, regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system are now widely used for genome editing in higher eukaryotic cells and whole organisms, revolutionising almost every discipline in biological research, medicine, and biotechnology. All of these nucleases, however, induce off-target mutations at sites homologous in sequence with on-target sites, limiting their utility in many applications including gene or cell therapy. In this review, we compare methods for detecting nuclease off-target mutations. We also review methods for profiling genome-wide off-target effects and discuss how to reduce or avoid offtarget mutations. (c) The Korean Society for Molecular and Cellular Biology. All rights reserved.
URI
https://pr.ibs.re.kr/handle/8788114/2351
ISSN
1016-8478
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
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