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뇌과학이미징연구단
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Bioorthogonal click chemistry-based synthetic cell glue

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Title
Bioorthogonal click chemistry-based synthetic cell glue
Author(s)
Heebeom Koo; Myunghwan Choi; Eunha Kim; Sei Kwang Hahn; Ralph Weissleder; Seok Hyun Yun
Publication Date
2015-12
Journal
SMALL, v.11, no.48, pp.6458 - 6466
Publisher
WILEY-V C H VERLAG GMBH
Abstract
Artificial methods of cell adhesion can be effective in building functional cell complexes in vitro, but methods for in vivo use are currently lacking. Here, a chemical cell glue based on bioorthogonal click chemistry with high stability and robustness is introduced. Tetrazine (Tz) and trans-cyclooctene (TCO) conjugated to the cell surface form covalent bonds between cells within 10 min in aqueous conditions. Glued, homogeneous, or heterogeneous cell pairs remain viable and stably attached in a microfluidic flow channel at a shear stress of 20 dyn cm−2. Upon intravenous injection of assembled Jurkat T cells into live mice, fluorescence microscopy shows the trafficking of cell pairs in circulation and their infiltration into lung tissues. These results demonstrate the promising potential of chemically glued cell pairs for various applications ranging from delivering therapeutic cells to studying cell–cell interactions in vivo.
URI
https://pr.ibs.re.kr/handle/8788114/2228
ISSN
1613-6810
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > Journal Papers (저널논문)
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