BROWSE

Related Scientist

Researcher

RNA 연구단
RNA 연구단
more info

Structure of human DROSHA Highly Cited Paper

Cited 51 time in webofscience Cited 0 time in scopus
514 Viewed 32 Downloaded
Title
Structure of human DROSHA
Author(s)
S. Chul Kwon; Tuan Anh Nguyen; Yeon-Gil Choi; Myung Hyun Jo; Sungchul Hohng; V. Narry Kim; Jae-Sung Woo
Publication Date
2016-01
Journal
CELL, v.164, no.1-2, pp.81 - 90
Publisher
CELL PRESS
Abstract
MicroRNA maturation is initiated by RNase III DROSHA that cleaves the stem loop of primary microRNA. DROSHA functions together with its cofactor DGCR8 in a heterotrimeric complex known as Microprocessor. Here, we report the X-ray structure of DROSHA in complex with the C-terminal helix of DGCR8. We find that DROSHA contains two DGCR8-binding sites, one on each RNase III domain (RIIID), which mediate the assembly of Microprocessor. The overall structure of DROSHA is surprisingly similar to that of Dicer despite no sequence homology apart from the C-terminal part, suggesting that DROSHA may have evolved from a Dicer homolog. DROSHA exhibits unique features, including non-canonical zinc-finger motifs, a long insertion in the first RIIID, and the kinked link between Connector helix and RIIID, which explains the 11-bp-measuring ‘‘ruler’’ activity of DROSHA. Our study implicates the evolutionary origin of DROSHA and elucidates the molecular basis of Microprocessor assembly and primary microRNA processing. ª2016 Elsevier Inc.
URI
https://pr.ibs.re.kr/handle/8788114/2219
ISSN
0092-8674
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
2016_Structure of human DROSHA.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse