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고규영
혈관 연구단
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Intravital imaging of intestinal lacteals unveils lipid drainage through contractility

Cited 34 time in webofscience Cited 33 time in scopus
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Title
Intravital imaging of intestinal lacteals unveils lipid drainage through contractility
Author(s)
K. Choe; Jang, J.Y.; Park, I.; Kim, Y.; Ahn, S.; Park, D.-Y.; Hong, Y.-K.; Alitalo, K.; Gou Young Koh; Kim, P.
Publication Date
2015-11
Journal
JOURNAL OF CLINICAL INVESTIGATION, v.125, no.11, pp.4042 - 4052
Publisher
AMER SOC CLINICAL INVESTIGATION INC
Abstract
Lacteals are lymphatic vessels located at the center of each intestinal villus and provide essential transport routes for lipids and other lipophilic molecules. However, it is unclear how absorbed molecules are transported through the lacteal. Here, we used reporter mice that express GFP under the control of the lymphatic-specific promoter Prox1 and a custom-built confocal microscope and performed intravital real-time visualization of the absorption and transport dynamics of fluorescence-tagged fatty acids (FAs) and various exogenous molecules in the intestinal villi in vivo. These analyses clearly revealed transepithelial absorption of these molecules via enterocytes, diffusive distribution over the lamina propria, and subsequent transport through lacteals. Moreover, we observed active contraction of lacteals, which seemed to be directly involved in dietary lipid drainage. Our analysis revealed that the smooth muscles that surround each lacteal are responsible for contractile dynamics and that lacteal contraction is ultimately controlled by the autonomic nervous system. These results indicate that the lacteal is a unique organ-specific lymphatic system and does not merely serve as a passive conduit but as an active pump that transports lipids. Collectively, using this efficient imaging method, we uncovered drainage of absorbed molecules in small intestinal villus lacteals and the involvement of lacteal contractibility. (c) The Journal of Clinical Investigation 2016
URI
http://pr.ibs.re.kr/handle/8788114/2204
DOI
10.1172/JCI76509
ISSN
0021-9738
Appears in Collections:
Center for Vascular Research(혈관 연구단) > Journal Papers (저널논문)
Files in This Item:
고-논문-2016.01-최기백-Intravital imaging of intestinal lacteals unveils lipid drainage through contractility.pdfDownload

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