Amyloid-Beta-Activated Human Microglial Cells Through ERResident Proteins

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Title
Amyloid-Beta-Activated Human Microglial Cells Through ERResident Proteins
Author(s)
Yong Cheol Yoo; Kyunghee Byun; Taewook Kang; Delger Bayarsaikhan; Jin Young Kim; Seyeoun Oh; Young Hye Kim; Se-Young Kim; Won-Il Chung; Seung U. Kim; Bonghee Lee; Young Mok Park
Publication Date
2015-01
Journal
JOURNAL OF PROTEOME RESEARCH, v.14, no.1, pp.214 - 233
Publisher
AMER CHEMICAL SOC
Abstract
Microglial activation in the central nervous system is a key event in the neuroinflammation that accompanies neurodegenerative diseases such as Alzheimer’s disease (AD). Among cytokines involved in microglial activation, amyloid β (Aβ) peptide is known to be a key molecule in the induction of diverse inflammatory products, which may lead to chronic inflammation in AD. However, proteomic studies of microglia in AD are limited due to lack of proper cell or animal model systems. In this study, we performed a proteomic analysis of Aβ-stimulated human microglial cells using SILAC (stable isotope labeling with amino acids in cell culture) combined with LC.MS/MS. Results showed that 60 proteins increased or decreased their abundance by 1.5 fold or greater. Among these, ER-resident proteins such as SERPINH1, PDIA6, PDIA3, and PPIB were revealed to be key molecular biomarkers of human microglial activation by validation of the proteomic results by immunostaining, PCR, ELISA, and Western blot. Taken together, our data suggest that ER proteins play an essential role in human microglial activation by Aβ and may be important molecular therapeutic targets for treatment of AD.
URI
https://pr.ibs.re.kr/handle/8788114/2140
ISSN
1535-3893
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
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