BROWSE

Related Scientist

Researcher

황대희
식물 노화·수명 연구단
more info

DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7R alpha(low) and IL-7R alpha(high) Effector Memory CD8(+) T Cells with Distinct Migratory Capacities to the Fractalkine

Cited 4 time in webofscience Cited 0 time in scopus
204 Viewed 52 Downloaded
Title
DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7R alpha(low) and IL-7R alpha(high) Effector Memory CD8(+) T Cells with Distinct Migratory Capacities to the Fractalkine
Author(s)
Shin, MS; You, S; Kang, YN; Lee, N; Yoo, SA; Park, K; Kang, KS; Kim, SH; Mohanty, S; Shaw, AC; Montgomery, RR; Daehee Hwang; Kang, I
Publication Date
2015-09
Journal
JOURNAL OF IMMUNOLOGY, v.195, no.6, pp.2861 - 2869
Publisher
AMER ASSOC IMMUNOLOGISTS
Abstract
DNA methylation is an epigenetic mechanism that modulates gene expression in mammalian cells including T cells. Memory T cells are heterogeneous populations. Human effector memory (EM) CD8(+) T cells in peripheral blood contain two cell subsets with distinct traits that express low and high levels of the IL-7R alpha. However, epigenetic mechanisms involved in defining such cellular traits are largely unknown. In this study, we use genome-wide DNA methylation and individual gene expression to show the possible role of DNA methylation in conferring distinct traits of chemotaxis and inflammatory responses in human IL-7R alpha(low) and IL-7R alpha(high) EM CD8(+) T cells. In particular, IL-7R alpha(low) EMCD8(+)T cells had increased expression of CX3CR1 along with decreased DNA methylation in the CX3CR1 gene promoter compared with IL-7R alpha(high) EM CD8(+) T cells. Altering the DNA methylation status of the CX3CR1 gene promoter changed its activity and gene expression. IL-7R alpha(low) EM CD8(+) T cells had an increased migratory capacity to the CX3CR1 ligand fractalkine compared with IL-7R alpha(high) EM CD8(+) T cells, suggesting an important biological outcome of the differential expression of CX3CR1. Moreover, IL-7R alpha(low) EM CD8(+) T cells induced fractalkine expression on endothelial cells by producing IFN-gamma and TNF-alpha, forming an autocrine amplification loop. Overall, our study shows the role of DNA methylation in generating unique cellular traits in human IL-7R alpha(low) and IL-7R alpha(high) EM CD8(+) T cells, including differential expression of CX3CR1, as well as potential biological implications of this differential expression
URI
https://pr.ibs.re.kr/handle/8788114/1881
ISSN
0022-1767
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > Journal Papers (저널논문)
Files in This Item:
13. DNA Methylation Regulates the Differential.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse