DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins Highly Cited Paper

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Title
DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
Author(s)
Je Wook Woo; Jungeun Kim; Soon Il Kwon; Claudia Corvalán; Seung Woo Cho; Hyeran Kim; Sang-Gyu Kim; Sang-Tae Kim; Sunghwa Choe; Jin-Soo Kim
Publication Date
2015-11
Journal
NATURE BIOTECHNOLOGY, v.33, no.11, pp.1162 - U156
Publisher
NATURE PUBLISHING GROUP
Abstract
Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation. © 2015 Nature America, Inc. All rights reserved
URI
https://pr.ibs.re.kr/handle/8788114/1840
ISSN
1087-0156
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
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