Integrated analysis of global proteome, phosphoproteome, and glycoproteome enables complementary interpretation of disease-related protein networks
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Title
- Integrated analysis of global proteome, phosphoproteome, and glycoproteome enables complementary interpretation of disease-related protein networks
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Author(s)
- Jong-Moon Park; Ji-Hwan Park; Dong-Gi Mun; Jingi Bae; Jae Hun Jung; Seunghoon Back; Hangyeore Lee; Hokeun Kim; Hee-Jung Jung; HarkKyun Kim; Hookeun Lee; Kwang Pyo Kim; Daehee Hwang; Sang-Won Lee
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Publication Date
- 2015-12
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Journal
- SCIENTIFIC REPORTS, v.5, pp.18189
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Publisher
- NATURE PUBLISHING GROUP
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Abstract
- Multi-dimensional proteomic analyses provide different layers of protein information, including
protein abundance and post-translational modifications. Here, we report an integrated analysis of
protein expression, phosphorylation, and N-glycosylation by serial enrichments of phosphorylation
and N-glycosylation (SEPG) from the same tissue samples. On average, the SEPG identified 142,106
unmodified peptides of 8,625 protein groups, 18,846 phosphopeptides (15,647 phosphosites),
and 4,019 N-glycopeptides (2,634 N-glycosites) in tumor and adjacent normal tissues from three
gastric cancer patients. The combined analysis of these data showed that the integrated analysis
additively improved the coverages of gastric cancer-related protein networks; phosphoproteome
and N-glycoproteome captured predominantly low abundant signal proteins, and membranous or
secreted proteins, respectively, while global proteome provided abundances for general population of
the proteome. Therefore, our results demonstrate that the SEPG can serve as an effective approach for
multi-dimensional proteome analyses, and the holistic profiles of protein expression and PTMs enabled
improved interpretation of disease-related networks by providing complementary information.
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URI
- https://pr.ibs.re.kr/handle/8788114/1828
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DOI
- 10.1038/srep18189
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ISSN
- 2045-2322
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Appears in Collections:
- Center for Plant Aging Research (식물 노화·수명 연구단) > 1. Journal Papers (저널논문)
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