Genome engineering in human cells

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Title
Genome engineering in human cells
Author(s)
Minjung Song; Young-Hoon Kim; Jin-Soo Kim; Hyongbum Kim
Publication Date
2014-11
Journal
METHODS IN ENZYMOLOGY, v.546, no.C, pp.93 - 118
Publisher
ELSEVIER ACADEMIC PRESS INC
Abstract
Genome editing in human cells is of great value in research, medicine, and biotechnology. Programmable nucleases including zinc-finger nucleases, transcription activator-like effector nucleases, and RNA-guided engineered nucleases recognize a specific target sequence and make a double-strand break at that site, which can result in gene disruption, gene insertion, gene correction, or chromosomal rearrangements. The target sequence complexities of these programmable nucleases are higher than 3.2 mega base pairs, the size of the haploid human genome. Here, we briefly introduce the structure of the human genome and the characteristics of each programmable nuclease, and review their applications in human cells including pluripotent stem cells. In addition, we discuss various delivery methods for nucleases, programmable nickases, and enrichment of gene-edited human cells, all of which facilitate efficient and precise genome editing in human cells. © 2014 Elsevier Inc. All rights reserved
URI
https://pr.ibs.re.kr/handle/8788114/1641
ISSN
0076-6879
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
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