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Imaging a specific mRNA in pollen with atomic force microscopy

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Title
Imaging a specific mRNA in pollen with atomic force microscopy
Author(s)
Kim J.S.; Park Y.S.; Hong Gil Nam; Park J.W.
Publication Date
2015
Journal
RSC ADVANCES, v.5, no.24, pp.18858 - 18865
Publisher
ROYAL SOC CHEMISTRY
Abstract
Intracellular localization of RNA is important in regulating various cellular functions. Here, we determined the intracellular localization of the ammonium transporter (AtAMT1;4) mRNA in the sperm cells of Arabidopsis pollen by atomic force microscopy (AFM). The AFM probe was modified with cone-shaped dendron molecules, and an oligonucleotide complementary to the mRNA was attached to the apex. This configuration allowed facile detection of the mRNA within a sectioned pollen cell at the single-molecule level by measuring the mRNA-oligonucleotide interaction force. The AtAMT1;4 mRNA was largely localized in the nucleus rather than the cytosol of the sperm cells
URI
https://pr.ibs.re.kr/handle/8788114/1639
ISSN
2046-2069
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > Journal Papers (저널논문)
Files in This Item:
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