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Bringing single-molecule spectroscopy to macromolecular protein complexes

Cited 18 time in webofscience Cited 17 time in scopus
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Title
Bringing single-molecule spectroscopy to macromolecular protein complexes
Author(s)
Chirlmin Joo; Mohamed Fareh; V. Narry Kim
Subject
single-molecule fluorescence, ; macromolecular complex, ; cell lysate, ; single-molecule immunoprecipitation, ; single-molecule pull-down, ; protein.
Publication Date
2013-01
Journal
TRENDS IN BIOCHEMICAL SCIENCES, v.38, no.1, pp.30 - 37
Publisher
ELSEVIER SCIENCE LONDON
Abstract
Single-molecule fluorescence spectroscopy offers realtime,nanometer-resolution information. Over the past two decades, this emerging single-molecule technique has been rapidly adopted to investigate the structural dynamics and biological functions of proteins. Despite this remarkable achievement, single-molecule fluorescence techniques must be extended to macromolecular protein complexes that are physiologically more relevant for functional studies. In this review, we present recent major breakthroughs for investigating protein complexes within cell extracts using single-molecule fluorescence. We outline the challenges, future prospects and potential applications of these new singlemolecule fluorescence techniques in biological and clinical research.
URI
https://pr.ibs.re.kr/handle/8788114/1602
DOI
10.1016/j.tibs.2012.10.005
ISSN
0968-0004
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
2013-01-Trendes in Biochemical Sciences-Bringing_single.pdfDownload

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