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Border Control-A Membrane-Linked Interactome of Arabidopsis

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Title
Border Control-A Membrane-Linked Interactome of Arabidopsis
Author(s)
Jones, AM; Xuan, YH; Xu, M; Wang, RS; Ho, CH; Lalonde, S; You, CH; Sardi, MI; Parsa, SA; Smith-Valle, E; Su, TY; Frazer, KA; Pilot, G; Pratelli, R; Grossmann, G; Acharya, BR; Hu, HC; Engineer, C; Villiers, F; Ju, CL; Takeda, K; Su, Z; Dong, QF; Assmann, SM; Chen, J; June Myoung Kwak; Schroeder, JI; Albert, R; Rhee, SY; Frommer, WB
Publication Date
2014-05
Journal
SCIENCE, v.344, no.6185, pp.711 - 716
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
Abstract
Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 106 pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, >99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split–green fluorescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms.
URI
https://pr.ibs.re.kr/handle/8788114/1518
ISSN
0036-8075
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > Journal Papers (저널논문)
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