Gene regulatory cascade of senescence-associated NAC transcription factors activated by ETHYLENE-INSENSITIVE2-mediated leaf senescence signalling in Arabidopsis
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- Gene regulatory cascade of senescence-associated NAC transcription factors activated by ETHYLENE-INSENSITIVE2-mediated leaf senescence signalling in Arabidopsis
- Hyojung Kim; Sunghyun Hong; You Wang Kim; Il Hwan Lee; Ji Hyung Jun; Bong Kwan Phee; Timilsina Rupak; Hana Jeong; Yeon Mi Lee; Byoung Seok Hong; Hong Gil Nam; Hye Ryun Woo; Pyung Ok Lim
- JOURNAL OF EXPERIMENTAL BOTANY, v.65, no.14, pp.4023 - 4036
- OXFORD UNIV PRESS
- Leaf senescence is a finely tuned and genetically programmed degeneration process, which is critical to maximize
plant fitness by remobilizing nutrients from senescing leaves to newly developing organs. Leaf senescence is a complex
process that is driven by extensive reprogramming of global gene expression in a highly coordinated manner.
Understanding how gene regulatory networks involved in controlling leaf senescence are organized and operated is
essential to decipher the mechanisms of leaf senescence. It was previously reported that the trifurcate feed-forward
pathway involving EIN2, ORE1, and miR164 in Arabidopsis regulates age-dependent leaf senescence and cell death.
Here, new components of this pathway have been identified, which enhances knowledge of the gene regulatory
networks governing leaf senescence. Comparative gene expression analysis revealed six senescence-associated
NAC transcription factors (TFs) (ANAC019, AtNAP, ANAC047, ANAC055, ORS1, and ORE1) as candidate downstream
components of ETHYLENE-INSENSITIVE2 (EIN2). EIN3, a downstream signalling molecule of EIN2, directly bound
the ORE1 and AtNAP promoters and induced their transcription. This suggests that EIN3 positively regulates leaf
senescence by activating ORE1 and AtNAP, previously reported as key regulators of leaf senescence. Genetic and
gene expression analyses in the ore1 atnap double mutant revealed that ORE1 and AtNAP act in distinct and overlapping
signalling pathways. Transient transactivation assays further demonstrated that ORE1 and AtNAP could activate
common as well as differential NAC TF targets. Collectively, the data provide insight into an EIN2-mediated senescence
signalling pathway that coordinates global gene expression during leaf senescence via a gene regulatory network
involving EIN3 and senescence-associated NAC TFs.
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