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나노물질 및 화학반응 연구단
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Volume-conserving trans-cis isomerization pathways in photoactive yellow protein visualized by picosecond X-ray crystallography

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dc.contributor.authorYang Ouk Jung-
dc.contributor.authorLee, Jae Hyuk-
dc.contributor.authorKim, Joonghan-
dc.contributor.authorSchmidt, Marius-
dc.contributor.authorMoffat, Keith-
dc.contributor.authorSrajer, Vukica-
dc.contributor.authorHyot Cherl Ihee-
dc.date.available2015-04-20T07:12:39Z-
dc.date.created2014-08-11-
dc.date.issued2013-03-
dc.identifier.issn1755-4330-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/1376-
dc.description.abstractTrans-to-cis isomerization, the key reaction in photoactive proteins, usually cannot occur through the standard one-bond-flip mechanism. Owing to spatial constraints imposed by a protein environment, isomerization probably proceeds through a volume-conserving mechanism in which highly choreographed atomic motions are expected, the details of which have not yet been observed directly. Here we employ time-resolved X-ray crystallography to visualize the isomerization of the p-coumaric acid chromophore in photoactive yellow protein with a time resolution of 100 ps and a spatial resolution of 1.6 Å. The structure of the earliest intermediate (IT) resembles a highly strained transition state in which the torsion angle is located halfway between the trans- and cis-isomers. The reaction trajectory of IT bifurcates into two structurally distinctcis intermediates via hula-twist and bicycle-pedal pathways. The bifurcating reaction pathways can be controlled by weakening the hydrogen bond between the chromophore and an adjacent residue through E46Q mutation, which switches off the bicycle-pedal pathway.-
dc.languageENG-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleVolume-conserving trans-cis isomerization pathways in photoactive yellow protein visualized by picosecond X-ray crystallography-
dc.typeArticle-
dc.type.rimsA-
dc.identifier.wosid000317182300013-
dc.identifier.scopusid2-s2.0-84875478146-
dc.description.wostc73-
dc.date.tcdate2018-10-01-
dc.date.scptcdate2018-10-01-
dc.contributor.affiliatedAuthorYang Ouk Jung-
dc.contributor.affiliatedAuthorHyot Cherl Ihee-
dc.identifier.bibliographicCitationNATURE CHEMISTRY, v.5, no.3, pp.212 - 220-
dc.description.scptc79-
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Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > Journal Papers (저널논문)
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