BROWSE

Related Scientist

rna's photo.

rna
rna연구단
more info

ITEM VIEW & DOWNLOAD

Quantitative and multiplexed microRNA sensing in living cells based on peptide nucleic acid and nano graphene oxide (PANGO)

Cited 205 time in webofscience Cited 211 time in scopus
1,815 Viewed 244 Downloaded
Title
Quantitative and multiplexed microRNA sensing in living cells based on peptide nucleic acid and nano graphene oxide (PANGO)
Author(s)
Ryoo S.-R.; Lee J.; Jinah Yeo; Na H.-K.; Kim Y.-K.; Jang H.; Lee J.H.; Han S.W.; Lee Y.; Vic Narry Kim; Dal-Hee Min
Subject
Fluorescence quenching, ; Graphene oxides, ; Peptide nucleic acid, ; Post-transcriptional, ; Quantitative monitoring, ; RNA recognition, ; Sequence specificity, ; Simultaneous monitoring, ; Biomolecules, ; Biosensors, ; Graphene, ; Loading, ; Nanotechnology, ; Peptides, ; Probes, ; Sensors, ; Transcription, ; RNA, ; graphite, ; microRNA, ; nanomaterial, ; oxide, ; peptide nucleic acid, ; article, ; chemistry, ; equipment, ; equipment design, ; equipment failure, ; genetic procedures, ; genetics, ; HeLa cell, ; human, ; materials testing, ; particle size, ; spectrofluorometry, ; surface property, ; ultrastructure, ; Biosensing Techniques, ; Equipment Design, ; Equipment Failure Analysis, ; Graphite, ; HeLa Cells, ; Humans, ; Materials Testing, ; Microchemistry, ; MicroRNAs, ; Nanostructures, ; Oxides, ; Particle Size, ; Peptide Nucleic Acids, ; Spectrometry, Fluorescence, ; Surface Properties
Publication Date
2013-07
Journal
ACS NANO, v.7, no.7, pp.5882 - 5891
Publisher
AMER CHEMICAL SOC
Abstract
MicroRNA (miRNA) is an important small RNA which regulates diverse gene expression at the post-transcriptional level. miRNAs are considered as important biomarkers since abnormal expression of specific miRNAs is associated with many diseases including cancer and diabetes. Therefore, it is important to develop biosensors to quantitatively detect miRNA expression levels. Here, we develop a nanosized graphene oxide (NGO) based miRNA sensor, which allows quantitative monitoring of target miRNA expression levels in living cells. The strategy is based on tight binding of NGO with peptide nucleic acid (PNA) probes, resulting in fluorescence quenching of the dye that is conjugated to the PNA, and subsequent recovery of the fluorescence upon addition of target miRNA. PNA as a probe for miRNA sensing offers many advantages including high sequence specificity, high loading capacity on the NGO surface compared to DNA and resistance against nuclease-mediated degradation. The present miRNA sensor allowed the detection of specific target miRNAs with the detection limit as low as ∼1 pM and the simultaneous monitoring of three different miRNAs in a living cell. © 2013 American Chemical Society.
URI
https://pr.ibs.re.kr/handle/8788114/1293
DOI
10.1021/nn401183s
ISSN
1936-0851
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
2013-07-23-ACS Nano-Quantitative_and_multiplexed_microRNA.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse