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Quantitative and multiplexed microRNA sensing in living cells based on peptide nucleic acid and nano graphene oxide (PANGO) Highly Cited Paper

Cited 143 time in webofscience Cited 0 time in scopus
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Title
Quantitative and multiplexed microRNA sensing in living cells based on peptide nucleic acid and nano graphene oxide (PANGO)
Author(s)
Ryoo S.-R.; Lee J.; Jinah Yeo; Na H.-K.; Kim Y.-K.; Jang H.; Lee J.H.; Han S.W.; Lee Y.; Vic Narry Kim; Dal-Hee Min
Publication Date
2013-07
Journal
ACS NANO, v.7, no.7, pp.5882 - 5891
Publisher
AMER CHEMICAL SOC
Abstract
MicroRNA (miRNA) is an important small RNA which regulates diverse gene expression at the post-transcriptional level. miRNAs are considered as important biomarkers since abnormal expression of specific miRNAs is associated with many diseases including cancer and diabetes. Therefore, it is important to develop biosensors to quantitatively detect miRNA expression levels. Here, we develop a nanosized graphene oxide (NGO) based miRNA sensor, which allows quantitative monitoring of target miRNA expression levels in living cells. The strategy is based on tight binding of NGO with peptide nucleic acid (PNA) probes, resulting in fluorescence quenching of the dye that is conjugated to the PNA, and subsequent recovery of the fluorescence upon addition of target miRNA. PNA as a probe for miRNA sensing offers many advantages including high sequence specificity, high loading capacity on the NGO surface compared to DNA and resistance against nuclease-mediated degradation. The present miRNA sensor allowed the detection of specific target miRNAs with the detection limit as low as ∼1 pM and the simultaneous monitoring of three different miRNAs in a living cell. © 2013 American Chemical Society.
URI
https://pr.ibs.re.kr/handle/8788114/1293
ISSN
1936-0851
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
2013-07-23-ACS Nano-Quantitative_and_multiplexed_microRNA.pdfDownload

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