IBS Publications Repository: Quantitative and multiplexed microRNA sensing in living cells based on peptide nucleic acid and nano graphene oxide (PANGO)

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Quantitative and multiplexed microRNA sensing in living cells based on peptide nucleic acid and nano graphene oxide (PANGO)

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Title: Quantitative and multiplexed microRNA sensing in living cells based on peptide nucleic acid and nano graphene oxide (PANGO)

Author(s): Ryoo S.-R.; Lee J.; Jinah Yeo; Na H.-K.; Kim Y.-K.; Jang H.; Lee J.H.; Han S.W.; Lee Y.; Vic Narry Kim; Dal-Hee Min

Subject: Fluorescence quenching, ; Graphene oxides, ; Peptide nucleic acid, ; Post-transcriptional, ; Quantitative monitoring, ; RNA recognition, ; Sequence specificity, ; Simultaneous monitoring, ; Biomolecules, ; Biosensors, ; Graphene, ; Loading, ; Nanotechnology, ; Peptides, ; Probes, ; Sensors, ; Transcription, ; RNA, ; graphite, ; microRNA, ; nanomaterial, ; oxide, ; peptide nucleic acid, ; article, ; chemistry, ; equipment, ; equipment design, ; equipment failure, ; genetic procedures, ; genetics, ; HeLa cell, ; human, ; materials testing, ; particle size, ; spectrofluorometry, ; surface property, ; ultrastructure, ; Biosensing Techniques, ; Equipment Design, ; Equipment Failure Analysis, ; Graphite, ; HeLa Cells, ; Humans, ; Materials Testing, ; Microchemistry, ; MicroRNAs, ; Nanostructures, ; Oxides, ; Particle Size, ; Peptide Nucleic Acids, ; Spectrometry, Fluorescence, ; Surface Properties

Abstract: MicroRNA (miRNA) is an important small RNA which regulates diverse gene expression at the post-transcriptional level. miRNAs are considered as important biomarkers since abnormal expression of specific miRNAs is associated with many diseases including cancer and diabetes. Therefore, it is important to develop biosensors to quantitatively detect miRNA expression levels. Here, we develop a nanosized graphene oxide (NGO) based miRNA sensor, which allows quantitative monitoring of target miRNA expression levels in living cells. The strategy is based on tight binding of NGO with peptide nucleic acid (PNA) probes, resulting in fluorescence quenching of the dye that is conjugated to the PNA, and subsequent recovery of the fluorescence upon addition of target miRNA. PNA as a probe for miRNA sensing offers many advantages including high sequence specificity, high loading capacity on the NGO surface compared to DNA and resistance against nuclease-mediated degradation. The present miRNA sensor allowed the detection of specific target miRNAs with the detection limit as low as ∼1 pM and the simultaneous monitoring of three different miRNAs in a living cell. © 2013 American Chemical Society.