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The RNA-binding protein repertoire of embryonic stem cells Highly Cited Paper

Cited 177 time in webofscience Cited 0 time in scopus
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Title
The RNA-binding protein repertoire of embryonic stem cells
Author(s)
S. Chul Kwon; Hyerim Yi; Eichelbaum, Katrin; Foehr, Sophia; Fischer, Bernd; Kwon Tae You; Castello, Alfredo; Krijgsveld, Jeroen; Hentze, Matthias W.; V. Narry Kim
Publication Date
2013-09
Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY, v.20, no.9, pp.1122 - 1130
Publisher
NATURE PUBLISHING GROUP
Abstract
RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and yet annotation of RBPs is limited mainly to those with known RNA-binding domains. To systematically identify the RBPs of embryonic stem cells (ESCs), we here employ interactome capture, which combines UV cross-linking of RBP to RNA in living cells, oligo(dT) capture and MS. From mouse ESCs (mESCs), we have defined 555 proteins constituting the mESC mRNA interactome, including 283 proteins not previously annotated as RBPs. Of these, 68 new RBP candidates are highly expressed in ESCs compared to differentiated cells, implicating a role in stem-cell physiology. Two well-known E3 ubiquitin ligases, Trim25 (also called Efp) and Trim71 (also called Lin41), are validated as RBPs, revealing a potential link between RNA biology and protein-modification pathways. Our study confirms and expands the atlas of RBPs, providing a useful resource for the study of the RNA-RBP network in stem cells. © 2013 Nature America, Inc. All rights reserved.
URI
https://pr.ibs.re.kr/handle/8788114/1267
ISSN
1545-9993
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
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