BROWSE

Related Scientist

cnir's photo.

cnir
뇌과학이미징연구단
more info

ITEM VIEW & DOWNLOAD

Protocol for mouse optogenetic fMRI at ultrahigh magnetic fields

Cited 0 time in webofscience Cited 0 time in scopus
189 Viewed 0 Downloaded
Title
Protocol for mouse optogenetic fMRI at ultrahigh magnetic fields
Author(s)
Hyun-Ji Shim; Geun Ho Im; Won Beom Jung; Hyun Seok Moon; Thi Ngoc Anh Dinh; Jeong-Yun Lee; Seong-Gi Kim
Publication Date
2022-12
Journal
STAR Protocols, v.3, no.4
Publisher
Cell Press
Abstract
Mouse optogenetic functional magnetic resonance imaging (opto-fMRI) is critical for linking genes and functions and for mapping cell-type-specific neural circuits in the whole brain. Herein, we describe how opto-fMRI images can be reliably obtained in anesthetized mice with minimal distortions at ultrahigh magnetic fields. The protocol includes surgical and anesthesia procedures, animal cradle modification, animal preparation and setup, animal physiology maintenance, and pilot fMRI scanning. This protocol will enable reproducible mouse opto-fMRI experiments. For complete details on the use and execution of this protocol, please refer to Jung et al. (2021),1 Jung et al. (2022),2 and Moon et al. (2021).3 © 2022 The Author(s)
URI
https://pr.ibs.re.kr/handle/8788114/12470
DOI
10.1016/j.xpro.2022.101846
ISSN
2666-1667
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse