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분자분광학및동력학연구단
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Label-Free Live-Cell Imaging of Internalized Microplastics and Cytoplasmic Organelles with Multicolor CARS Microscopy

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dc.contributor.authorChoi, Dae Sik-
dc.contributor.authorSohee Lim-
dc.contributor.authorJin-Sung Park-
dc.contributor.authorKim, Chang-Ho-
dc.contributor.authorRhee, Hanju-
dc.contributor.authorMinhaeng Cho-
dc.date.accessioned2022-05-25T04:46:30Z-
dc.date.available2022-05-25T04:46:30Z-
dc.date.created2022-03-02-
dc.date.issued2022-03-
dc.identifier.issn0013-936X-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/11585-
dc.description.abstract© 2022 American Chemical Society.As the bioaccumulation of microplastics (MPs) is considered as a potential health risk, many efforts have been made to understand the cellular dynamics and cytotoxicity of MPs. Here, we demonstrate that label-free multicolor coherent anti-Stokes Raman scattering (CARS) microscopy enables separate vibrational imaging of internalized MPs and lipid droplets (LDs) with indistinguishable shapes and sizes in live cells. By simultaneously obtaining polystyrene (PS)- and lipid-specific CARS images at two very different frequencies, 1000 and 2850 cm-1, respectively, we successfully identify the local distribution of ingested PS beads and native LDs in Caenorhabditis elegans. We further show that the movements of PS beads and LDs in live cells can be separately tracked in real time, which allows us to characterize their individual intracellular dynamics. We thus anticipate that our multicolor CARS imaging method could be of great use to investigate the cellular transport and cytotoxicity of MPs without additional efforts for pre-labeling to MPs.-
dc.language영어-
dc.publisherAmerican Chemical Society-
dc.titleLabel-Free Live-Cell Imaging of Internalized Microplastics and Cytoplasmic Organelles with Multicolor CARS Microscopy-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000776699100017-
dc.identifier.scopusid2-s2.0-85124908667-
dc.identifier.rimsid77762-
dc.contributor.affiliatedAuthorSohee Lim-
dc.contributor.affiliatedAuthorJin-Sung Park-
dc.contributor.affiliatedAuthorMinhaeng Cho-
dc.identifier.doi10.1021/acs.est.1c06255-
dc.identifier.bibliographicCitationEnvironmental Science and Technology, v.56, no.5, pp.3045 - 3055-
dc.relation.isPartOfEnvironmental Science and Technology-
dc.citation.titleEnvironmental Science and Technology-
dc.citation.volume56-
dc.citation.number5-
dc.citation.startPage3045-
dc.citation.endPage3055-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaEngineering-
dc.relation.journalResearchAreaEnvironmental Sciences & Ecology-
dc.relation.journalWebOfScienceCategoryEngineering, Environmental-
dc.relation.journalWebOfScienceCategoryEnvironmental Sciences-
dc.subject.keywordPlusRAMAN-SPECTROSCOPY-
dc.subject.keywordPlusMULTIPLEX CARS-
dc.subject.keywordPlusIN-VIVO-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusMICROSPECTROSCOPY-
dc.subject.keywordPlusPARTICLES-
dc.subject.keywordPlusSIZE-
dc.subject.keywordPlusMICROPARTICLES-
dc.subject.keywordPlusNANOPARTICLES-
dc.subject.keywordPlusABSORPTION-
dc.subject.keywordAuthorlabel-free live-cell imaging-
dc.subject.keywordAuthorlipid droplet-
dc.subject.keywordAuthormicroplastic internalization-
dc.subject.keywordAuthormulticolor CARS microscopy-
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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