BROWSE

Related Scientist

GEL's photo.

GEL
유전체 교정 연구단
more info

ITEM VIEW & DOWNLOAD

Generation of a Dystrophin Mutant in Dog by Nuclear Transfer Using CRISPR/Cas9-Mediated Somatic Cells: A Preliminary Study

Cited 0 time in webofscience Cited 0 time in scopus
180 Viewed 0 Downloaded
Title
Generation of a Dystrophin Mutant in Dog by Nuclear Transfer Using CRISPR/Cas9-Mediated Somatic Cells: A Preliminary Study
Author(s)
Oh, Hyun Ju; Eugene Chung; Kim, Jaehwan; Kim, Min Jung; Kim, Geon A.; Lee, Seok Hee; Ra, Kihae; Eom, Kidong; Park, Soojin; Chae, Jong-Hee; Jin-Soo Kim; Lee, Byeong Chun
Publication Date
2022-03
Journal
International Journal of Molecular Sciences, v.23, no.5
Publisher
MDPI
Abstract
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.Dystrophinopathy is caused by mutations in the dystrophin gene, which lead to progressive muscle degeneration, necrosis, and finally, death. Recently, golden retrievers have been suggested as a useful animal model for studying human dystrophinopathy, but the model has limitations due to difficulty in maintaining the genetic background using conventional breeding. In this study, we suc-cessfully generated a dystrophin mutant dog using the CRISPR/Cas9 system and somatic cell nuclear transfer. The dystrophin mutant dog displayed phenotypes such as elevated serum creatine kinase, dystrophin deficiency, skeletal muscle defects, an abnormal electrocardiogram, and avoidance of am-bulation. These results indicate that donor cells with CRISPR/Cas9 for a specific gene combined with the somatic cell nuclear transfer technique can efficiently produce a dystrophin mutant dog, which will help in the successful development of gene therapy drugs for dogs and humans.
URI
https://pr.ibs.re.kr/handle/8788114/11583
DOI
10.3390/ijms23052898
ISSN
1661-6596
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse