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A Phosphate-Binding Pocket within the Platform-PAZ-Connector Helix Cassette of Human Dicer

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Title
A Phosphate-Binding Pocket within the Platform-PAZ-Connector Helix Cassette of Human Dicer
Author(s)
Yuan Tian; Dhirendra K. Simanshu; Jin-Biao Ma; Jong-Eun Park; Inha Heo; V. Narry Kim; Dinshaw J. Patel
Publication Date
2014-02
Journal
MOLECULAR CELL, v.53, no.4, pp.606 - 616
Publisher
CELL PRESS
Abstract
We have solved two families of crystal structures of the human Dicer ‘‘platform-PAZ-connector helix’’cassette in complex with small interfering RNAs(siRNAs). The structures possess two adjacently positioned pockets: a 2 nt 30-overhang-binding pocket within the PAZ domain (30 pocket) and a phosphate-binding pocket within the platform domain(phosphate pocket). One family of complexes contains a knob-like a-helical protrusion, designated ‘‘hDicer-specific helix,’’ that separates the two pockets and orients the bound siRNA away from the surface of Dicer, which could be indicative of a product release/transfer state. In the second complex, the helical protrusion is melted/disordered and the bound siRNA is aligned toward the surface of Dicer, suggestive of a cleavage-competent state. These structures allow us to propose that the transition from the cleavage-competent to the postulated product release/transfer state may involve release of the 50-phosphate from the phosphate pocket while retaining the 30 overhang in the 30 pocket.
URI
https://pr.ibs.re.kr/handle/8788114/1121
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
38. molecular Cell - A Phosphate-Binding.pdfDownload

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