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TAIL-seq: Genome-wide determination of poly(A) tail length and 3' end modifications Highly Cited Paper

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Title
TAIL-seq: Genome-wide determination of poly(A) tail length and 3' end modifications
Author(s)
Hyeshik Chang; Jaechul Lim; Minju Ha; V. Narry Kim
Publication Date
2014-03
Journal
MOLECULAR CELL, v.53, no.6, pp.1044 - 1052
Publisher
CELL PRESS
Abstract
Global investigation of the 30 extremity of mRNA (30-terminome), despite its importance in gene regulation,has not been feasible due to technical challenges associated with homopolymeric sequences and relative paucity of mRNA. We here develop a method, TAIL-seq, to sequence the very end of mRNA molecules. TAIL-seq allows us to measure poly(A) tail length at the genomic scale. Median poly(A) length is 50–100 nt in HeLa and NIH 3T3 cells. Poly(A) length correlates with mRNA half-life, but not with translational efficiency. Surprisingly, we discover widespread uridylation and guanylation at the downstream of poly(A) tail. The U tails are generally attached to short poly(A) tails (<25 nt), while the G tails are found mainly on longer poly(A) tails (>40 nt), implicating their generic roles in mRNA stability control. TAIL-seq is a potent tool to dissect dynamic control of mRNA turnover and translational control, and to discover unforeseen features of RNA cleavage and tailing.
URI
https://pr.ibs.re.kr/handle/8788114/1096
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
37. molecular Cell - TAIL-seq.pdfDownload

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