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Kim, Dae Sik
유전체 교정 연구단
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Profiling genome-wide specificity of CRISPR-Cas9 using digenome-seq

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Title
Profiling genome-wide specificity of CRISPR-Cas9 using digenome-seq
Author(s)
Daesik Kim; Jin-Soo Kim
Publication Date
2021-01
Journal
Methods in molecular biology (Clifton, N.J.), v.2162, pp.233 - 242
Publisher
Humana Press, Inc.
Abstract
Digenome-seq is a highly sensitive method for analyzing the genome-wide specificity of CRISPR-Cas9 nuclease activity. In this procedure, genomic DNA is first subjected to digestion by CRISPR-Cas9 in vitro and then to whole genome sequencing, which results in unusual patterns of straight alignments at on-target and potential off-target sites. Analysis of these data with the Digenome-seq computer program allows for identification of the in vitro cleavage sites associated with the straight alignments. Here, we present a detailed Digenome-seq protocol for genome-wide profiling of in vitro CRISPR-Cas9 nuclease cleavage sites
URI
https://pr.ibs.re.kr/handle/8788114/10910
DOI
10.1007/978-1-0716-0687-2_13
ISSN
1064-3745
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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