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Choi, Minkyung
유전체 교정 연구단
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The functional association of acqos/victr with salt stress resistance in arabidopsis thaliana was confirmed by crispr-mediated mutagenesis

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Title
The functional association of acqos/victr with salt stress resistance in arabidopsis thaliana was confirmed by crispr-mediated mutagenesis
Author(s)
Kim, Sang-Tae; Minkyung Choi; Su-Ji Bae; Jin-Soo Kim
Publication Date
2021-11
Journal
International Journal of Molecular Sciences, v.22, no.21
Publisher
MDPI
Abstract
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.Clustered regularly interspaced palindromic repeat (CRISPR)-mediated mutagenesis has become an important tool in plant research, enabling the characterization of genes via gene knock-out. CRISPR genome editing tools can be applied to generate multi-gene knockout lines. Typically, multiple single-stranded, single guide RNAs (gRNAs) must be expressed in an organism to target multiple genes simultaneously; however, a single gRNA can target multiple genes if the target genes share similar sequences. A gene cluster comprising ACQUIRED OSMOTOLERANCE (ACQOS; AT5G46520) and neighboring nucleotide-binding leucine-rich repeats (NLRs; AT5G46510) is associated with osmotic tolerance. To investigate the role of ACQOS and the tandemly arranged NLR in osmotic tolerance, we introduced small insertion/deletion mutations into two target genes using a single gRNA and obtained transformant plant lines with three different combinations of mutant alleles. We then tested our mutant lines for osmotic tolerance after a salt-stress acclimation period by determining the chlorophyll contents of the mutant seedlings. Our results strongly suggest that ACQOS is directly associated with salt resistance, while the neighboring NLR is not. Here, we confirmed previous findings suggesting the involvement of ACQOS in salt tolerance and demonstrated the usefulness of CRISPR-mediated mutagenesis in validating the functions of genes in a single genetic background.
URI
https://pr.ibs.re.kr/handle/8788114/10715
DOI
10.3390/ijms222111389
ISSN
1661-6596
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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