A guide to genome engineering with programmable nucleases Highly Cited Paper

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Title
A guide to genome engineering with programmable nucleases
Author(s)
Hyongbum Kim; Jin-Soo Kim
Publication Date
2014-05
Journal
NATURE REVIEWS GENETICS, v.15, no.5, pp.321 - 334
Publisher
NATURE PUBLISHING GROUP
Abstract
Programmable nucleases — including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived from the bacterial clustered regularly interspaced short palindromic repeat (CRISPR)–Cas (CRISPR-associated) system — enable targeted genetic modifications in cultured cells, as well as in whole animals and plants. The value of these enzymes in research, medicine and biotechnology arises from their ability to induce site-specific DNA cleavage in the genome, the repair (through endogenous mechanisms) of which allows high-precision genome editing. However, these nucleases differ in several respects, including their composition, targetable sites, specificities and mutation signatures, among other characteristics. Knowledge of nuclease-specific features, as well as of their pros and cons, is essential for researchers to choose the most appropriate tool for a range of applications.
URI
https://pr.ibs.re.kr/handle/8788114/1044
ISSN
1471-0056
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
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