BROWSE

Related Scientist

Researcher

면역미생물공생연구단
면역 미생물 공생 연구단
more info

UNC93B1 is essential for the plasma membrane localization and signaling of Toll-like receptor 5

Cited 19 time in webofscience Cited 0 time in scopus
351 Viewed 21 Downloaded
Title
UNC93B1 is essential for the plasma membrane localization and signaling of Toll-like receptor 5
Author(s)
Huh, J.W.; Shibata, T.; Hwang, M.; Kwon, E.H.; Min Seong Jang; Fukui, R.; Kanno, A.; Jung, D.J.; Myoung Ho Jang; Miyake, K.; Kim, Y.M.
Publication Date
2014-05
Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.111, no.19, pp.7072 - 7077
Publisher
NATL ACAD SCIENCES
Abstract
The proper trafficking and localization of Toll-like receptors (TLRs) are important for specific ligand recognition and efficient signal transduction. The TLRs sensing bacterial membrane components are expressed on the cell surface and recruit signaling adaptors to the plasma membrane upon stimulation. On the contrary, the nucleotide-sensing TLRs are mostly found inside cells and signal from the endolysosomes in an acidic pH-dependent manner. Trafficking of the nucleotide-sensing TLRs from the endoplasmic reticulum to the endolysosomes strictly depends on UNC93B1, and their signaling is completely abolished in the 3d mutant mice bearing the H412R mutation of UNC93B1. In contrast, UNC93B1 was considered to have no role for the cell surface-localized TLRs and signaling via TLR1, TLR2, TLR4, and TLR6 is normal in the 3d mice. Unexpectedly, we discovered that TLR5, a cell surface receptor for bacterial protein flagellin, also requires UNC93B1 for plasma membrane localization and signaling. TLR5 physically interacts with UNC93B1, and the cells from the 3d or UNC93B1- deficient mice not only lack TLR5 at the plasma membrane but also fail to secret cytokines and to up-regulate costimulatory molecules upon flagellin stimulation, demonstrating the essential role of UNC93B1 in TLR5 signaling. Our study reveals that the role of UNC93B1 is not limited to the TLRs signaling from the endolysosomes and compels the further probing of the mechanisms underlying the UNC93B1-assisted differential targeting of TLRs.
URI
https://pr.ibs.re.kr/handle/8788114/1039
ISSN
0027-8424
Appears in Collections:
Academy of Immunology and Microbiology(면역 미생물 공생 연구단) > Journal Papers (저널논문)
Files in This Item:
첨부4. UNC93B1 is essential for the plasma membrane localization and signaling of Toll-like receptor 5.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse