JOURNAL OF MATERIALS CHEMISTRY B, v.2, no.17, pp.2452 - 2460
ROYAL SOC CHEMISTRYROYAL SOC CHEMISTRY
Graphene oxide (GO), an oxidized 2-dimensional carbon material, has been studied actively in both
academia and industry due to its unique physicochemical properties including high surface area,
excellent aqueous dispersibility, quenching capability of contiguous fluorophores and adsorption affinity
with diverse biomolecules. Particularly, GO has been widely used in biological applications involving
small molecule biosensors, enzymatic reaction monitoring, drug delivery and therapeutic applications.
Among these rapidly developing fields, we focused on the current advancements of fluorescence
mediated activity assay platforms for enzymes including nucleases, methyltransferases, protein kinases,
caspases and helicases by using affinity and fluorescence quenching capability of GO with fluorophore
labelled biomolecule substrates in the aqueous solution phase, and its important applications with future
perspectives in this article.