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Spc1 regulates the signal peptidase-mediated processing of membrane proteins

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Title
Spc1 regulates the signal peptidase-mediated processing of membrane proteins
Author(s)
Yim, Chewon; Chung, Yeonji; Jeesoo Kim; Nilsson, Ingmarie; Jong-Seo Kim; Kim, Hyun
Subject
INTERNAL SIGNAL, ; CLEAVAGE, ; COMPLEX, ; SEC11, ; ANCHOR, ; PURIFICATION, ; SEQUENCES, ; CONTAINS, ; PROLINE, ; SUBUNIT
Publication Date
2021-07
Journal
JOURNAL OF CELL SCIENCE, v.134, no.13
Publisher
Company of Biologists Ltd
Abstract
© 2021. Published by The Company of Biologists Ltd.Signal peptidase (SPase) cleaves the signal sequences (SSs) of secretory precursors. It contains an evolutionarily conserved membrane protein subunit, Spc1, that is dispensable for the catalytic activity of SPase and whose role remains unknown. In this study, we investigated the function of yeast Spc1. First, we set up an in vivo SPase cleavage assay using variants of the secretory protein carboxypeptidase Y (CPY) with SSs modified in the N-terminal and hydrophobic core regions. When comparing the SS cleavage efficiencies of these variants in cells with or without Spc1, we found that signal-anchored sequences became more susceptible to cleavage by SPase without Spc1. Furthermore, SPase-mediated processing of model membrane proteins was enhanced in the absence of Spc1 and was reduced upon overexpression of Spc1. Spc1 co-immunoprecipitated with proteins carrying uncleaved signal-anchored or transmembrane (TM) segments. Taken together, these results suggest that Spc1 protects TM segments from SPase action, thereby sharpening SPase substrate selection and acting as a negative regulator of the SPase-mediated processing of membrane proteins.
URI
https://pr.ibs.re.kr/handle/8788114/10175
DOI
10.1242/jcs.258936
ISSN
0021-9533
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
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