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µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo

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Title
µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo
Author(s)
Jisoo Han; Choi, Pyonggang; Choi, Myunghwan
Publication Date
2021-04
Journal
Journal of Visualized Experiments, no.170
Publisher
Journal of Visualized Experiments
Abstract
Intravital fluorescence microscopy is a tool used widely to study multicellular dynamics in a live animal. However, it has not been successfully used in the taste sensory organ. By integrating microfluidics into the intravital tongue imaging window, the µTongue provides reliable functional images of taste cells in vivo under controlled exposure to multiple tastants. In this paper, a detailed step-by-step procedure to utilize the µTongue system is presented. There are five subsections: preparing of tastant solutions, setting up of a microfluidic module, sample mounting, acquiring functional image data, and data analysis. Some tips and techniques to solve the practical issues that may arise when using the µTongue are also presented.
URI
https://pr.ibs.re.kr/handle/8788114/10050
DOI
10.3791/62361
ISSN
1940-087X
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > 1. Journal Papers (저널논문)
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